期刊
ACTA BIOMATERIALIA
卷 10, 期 6, 页码 2518-2528出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2013.12.052
关键词
Bone tissue engineering; Hydrogel; LIPUS; Osteogenic differentiation; Endothelial differentiation
资金
- National Basic Research Program of China (973 Program) [2012CB933600]
- National Natural Science Foundation of China [51072167, 31370966]
- Health Department of Sichuan Province [110281]
- Fundamental Research Funds for the Central Universities [SWJTU11ZT05]
The level of formation of new bone and vascularization in bone tissue engineering scaffold implants is considered as a critical factor for clinical application. In this study, an approach using an RGD-grafted oxidized sodium alginate/N-succinyl chitosan (RGD-OSA/NSC) hydrogel as a scaffold and low-intensity pulsed ultrasound (LIPUS) as mechanical stimulation was proposed to achieve a high level of formation of new bone and vascularization. An in vitro study of endothelial and osteogenic differentiations of human-bone-marrow-derived mesenchymal stem cells (hMSCs) was conducted to evaluate it. The results showed that RGD-OSA/NSC composite hydrogels presented good biological properties in attachment, proliferation and differentiation of cells. The MTT cell viability assay showed that the total number of cells increased more significantly in the LIPUS-stimulated groups with RGD than that in the control ones; similar results were obtained for alkaline phosphatase activity/staining and mineralized nodule formation assay of osteogenic induction and immunohistochemical test of endothelial induction. The positive synergistic effect of LIPUS and RGD on the enhancement of proliferation and differentiation of hMSCs was observed. These findings suggest that the hybrid use of RGD modification and LIPUS might provide one approach to achieve a high level of formation of new bone and vascularization in bone tissue engineering scaffold implants. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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