期刊
ACTA BIOMATERIALIA
卷 10, 期 10, 页码 4425-4436出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2014.06.023
关键词
Collagen hydrogel; Carbon nanotubes; Mesenchymal stem cells; Neurotrophic factors; Neural regeneration
资金
- Priority Research Centers Program [2009-0093829]
- Basic Science Research Program National Research Foundation, South Korea [2013R1A1A4A01012572]
- National Research Foundation of Korea [2013R1A1A4A01012572] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Microenvironments provided by three-dimensional (3-D) hydrogels mimic native tissue conditions, supplying appropriate physical cues for regulating stem cell behaviors. Here, we focused on carbon nanotubes (CNTs) dispersed within collagen hydrogels to provide 3-D microenvironmental conditions for mesenchymal stem cells (MSCs) in stimulating biological functions for neural regeneration. Small concentrations of CNTs (0.1-1 wt.%) did not induce toxicity to MSCs, and even improved the proliferative potential of the cells. MSCs cultured within the CNT-collagen hydrogel expressed considerable levels of neural markers, including GAP43 and pm tubulin proteins by immunostaining as well as GAP43 and synapse I genes by reverse transcriptase polymerase chain reaction (RT-PCR). Of note was that neurotrophic factors, particularly nerve growth factor and brain derived neurotrophic factor, were significantly promoted by the incorporation of CNTs as confirmed by RT-PCR and Western blot analysis. A model experiment involving neuritogenesis of PC12 cells influenced by those releasing neurotrophic factors from MSCs cultured within the CNT-collagen hydrogel demonstrated the significant enhancement in neurite outgrowth behaviors. Taken together, collagen hydrogel provides excellent 3-D conditions for MSC growth, and a small incorporation of CNTs within the hydrogel significantly stimulates MSC expression of neural markers and secretion of neurotrophic factors. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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