期刊
ACTA BIOMATERIALIA
卷 10, 期 12, 页码 4947-4955出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2014.06.031
关键词
Transgenic silkworm; Recombinant human acidic fibroblast growth factor; Silk; Biomedical material
资金
- National Basic Research Program of China (973 program) [2012CB114600]
- National Hi-Tech Research and Development Program of China (863 program) [2011AA100306]
- doctoral innovation fund of southwest university [kb2011008]
Natural silk fiber spun by the silkworm Bombyx mori is widely used not only for textile materials, but also for biofunctional materials. In the present study, we genetically engineered an advanced silk material, named hSFSV, using a transgenic silkworm, in which the recombinant human acidic fibroblast growth factor (hFGF1) protein was specifically synthesized in the middle silk gland and secreted into the sericin layer to surround the silk fiber using our previously optimized sericin1 expression system. The content of the recombinant hFGF1 in the hSFSV silk was estimated to be approximate 0.07% of the cocoon shell weight. The mechanical properties of hSFSV raw silk fiber were enhanced slightly compared to those of the wild-type raw silk fiber, probably due to the presence of the recombinant of hFGF1 in the sericin layer. Remarkably, the hSFSV raw silk significantly stimulated the cell growth and proliferation of NIH/3T3 mouse embryonic fibroblast cells, suggesting that the mitogenic activity of recombinant hFGF1 was well maintained and functioned in the sericin layer of hSFSV raw silk. These results show that the genetically engineered raw silk hSFSV could be used directly as a fine biomedical material for mass application. In addition, the strategy whereby functional recombinant proteins are expressed in the sericin layer of silk might be used to create more genetically engineered silks with various biofunctions and applications. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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