期刊
ACTA BIOMATERIALIA
卷 10, 期 2, 页码 732-741出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2013.09.041
关键词
Quantum dot; Cadmium; Toxicity; Degradation; Particle functionality
资金
- FWO-Vlaanderen (Krediet aan Navorsers)
- UGent consortium NB Photonics
- European Commission
Colloidal semiconductor nanoparticles (quantum dots) have attracted a lot of interest in technological and biomedical research, given their potent fluorescent properties. However, the use of heavy-metal-containing nanoparticles remains an issue of debate. The possible toxic effects of quantum dots remain a hot research topic and several questions such as possible intracellular degradation of quantum dots and the effect thereof on both cell viability and particle functionality remain unresolved. In the present work, poly(methacrylic acid)-coated CdSe/ZnS quantum dots were synthesized and characterized, after which their effects on cultured cells were evaluated using a multiparametric setup. The data reveal that the quantum dots are taken up through endocytosis and when exposed to the low pH of the endosomal structures, they partially degrade and release cadmium ions, which lowers their fluorescence intensity and augments particle toxicity. Using the multiparametric method, the quantum dots were evaluated at non-toxic doses in terms of their ability to visualize labeled cells for longer time periods. The data revealed that comparing different particles in terms of their applied dose is challenging, likely due to difficulties in obtaining accurate nanoparticle concentrations, but evaluating particle toxicity in terms of their biological functionality enables an easy and straightforward comparison. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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