期刊
ACTA BIOMATERIALIA
卷 10, 期 3, 页码 1451-1462出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2013.12.012
关键词
Hydroxyapatite; Lithium; Osseointegration; Gene expression; Wnt signaling pathway
资金
- Swedish Research Council (VR) [K20125-2X-094952-53, 6212-0116-037]
- ALF/LUA
- BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy
- VINNOVA
- Region Vastra Gotaland
Owing to its bio- and osteoconductivity, hydroxyapatite (HA) is a widely used implant material, but its osteogenic properties are only partly evaluated in vitro and in vivo. The present study focused on bone healing adjacent to HA-coated titanium (Ti) implants, with or without incorporated lithium ions (Li+). Special attention was given to the Wnt signaling pathway. The implants were inserted into rat tibia for 7 or 28 days and analyzed ex vivo, mainly by histomorphometry and quantitative real-time polymerase chain reaction (qPCR). HA-coated implants showed, irrespective of Li+ content, bone-implant contact (BIC) and removal torque values significantly higher than those of reference Ti. Further, the expression of OCN, CTSK, COL1A1, LRP5/6 and WISP1 was significantly higher in implant-adherent cells of HA-coated implants, with or without Li+. Significantly higher beta-catenin expression and significantly lower COL2A1 expression were observed in pen-implant bone cells from HA with 14 ng cm(-2) released Li+. Interestingly, Ti implants showed a significantly larger bone area (BA) in the threads than HA with 39 ng cm(-2) released Li+, but had a lower BIC than any HA-coated implant. This study shows that HA, with or without Li+, is a strong activator of the Wnt signaling pathway, and may to some degree explain its high bone induction capacity. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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