期刊
ACTA BIOMATERIALIA
卷 10, 期 11, 页码 4912-4918出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2014.07.025
关键词
Aseptic loosening; Wear particle; Strontium ranelate; RANKL; TNF-alpha
资金
- Jiangsu Province's Key Medical center, Jiangsu Provincial Special Program of Medical Science [BL2012004]
- National Nature Science Foundation of China [81372018, 81272018, 81101399]
- Nature Science Foundation of Jiangsu Province [BK2011303]
- Project of Jiangsu College graduate research and innovation program [CXZZ13_0835]
Wear-particle-induced osteolysis is considered to be the main reason for revision after arthroplasty. Although the exact mechanism remains unclear, inflammatory osteoclastogenesis plays an important role in this process. Strontium ranelate (SR) was found to have a therapeutic effect on osteoporosis in postmenopausal women. Based on prior studies, the present authors hypothesized that SR prevents wear-particle-induced osteolysis through restraining inflammatory osteoclastogenesis. The present study used 80 male C57BL/J6 mice to test this hypothesis in a murine osteolysis model. All experimental animals were randomly divided into four groups: a control group; a SR group; a titanium group; and a titanium + SR group. Once titanium particles had been implanted in mice, the mice were administered SR (900 mg kg(-1) day(-1)) by gavage for 14 days. After 14 days, the calvaria were collected for micro-computed tomography (mu CT), histological and molecular analysis. The results of mu CT and histomorphometric analysis demonstrated that SR markedly inhibited bone resorption and the generation of tartrate-resistant acid-phosphatase-positive cells in vivo, compared with titanium-stimulated calvaria. Reverse transcription polymerase chain reaction and ELISAs showed that SR stimulated the mRNA and protein expression of osteoprotegerin, and inhibited gene and protein expression of receptor activators of nuclear factor-kappa B ligand in titanium-particle-charged calvaria. In addition, SR obviously reduced the secretion of tumor necrosis factor-a and interleukin-1 beta in the calvaria of the titanium group. It was concluded that SR inhibits titanium-induced osteolysis by restraining inflammatory osteoclastogenesis, and that it could be developed as a new drug to prevent and treat aseptic loosening. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据