期刊
ACTA BIOMATERIALIA
卷 9, 期 1, 页码 4592-4598出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2012.08.039
关键词
Cell micropatterning; Retinoic acid; Neurite outgrowth; Cell and nucleus orientation; Neuronal regenerative medicine
资金
- AO Foundation Research [S-10-7L]
- NE DHHS Stem Cell Research Grant [2011-05]
- Army-UNL Center for Trauma Mechanics [W911NF-11-1-0033]
Creating physical-biochemical superposed microenvironments optimal for stimulating neurite outgrowth would be beneficial for neuronal regenerative medicine. We investigated potential co-regulatory effects of cell micropatteming and retinoic acid (RA) soluble factor on neuronal cell morphology and neurite outgrowth. Human neuroblastoma (SH-SY5Y) cell patterning sensitivity could be enhanced by poly-L-lysine-g-polyethylene glycol cell-repellent back-filling, enabling cell confinement in lanes as narrow as 5 mu m. Cells patterned on narrow (5 and 10 mu m) lanes showed preferred nucleus orientation following the patterning direction. These cells also showed high nucleus aspect ratio but constrained nucleus spreading. On the other hand, cells on wide (20 mu m and above) lanes showed random nucleus orientation and cell and nucleus sizes similar to those on unpatterned controls. All these changes were generally maintained with or without RA. Confining cells on narrow (5 and 10 mu m) lanes, even without RA, significantly enhanced neurite extension relative to unpatterned control, which was further stimulated by RA. Interestingly, cell patterning on 5 and 10 mu m lanes without RA produced longer neurites relative to the RA treatment alone case. Our data on the potential interplay between microscale physical cell confinement and RA-soluble stimulation may provide a new, integrative insight on how to trigger neurite/axon formation for neuronal regenerative medicine. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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