期刊
ACTA BIOMATERIALIA
卷 9, 期 11, 页码 8921-8931出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2013.06.035
关键词
Decellularization; Adipose tissue extracellular matrix; Adipose-derived stem cells; Fat grafting; Adipose tissue engineering
资金
- MD Anderson High Resolution Electron Microscopy Facility (NIH Cancer Center core grant) [CA16672]
- Kyte Foundation through the Department of Plastic Surgery of MD Anderson Cancer Center
Repair of soft tissue defects resulting from lumpectomy or mastectomy has become an important rehabilitation process for breast cancer patients. This study aimed to provide an adipose tissue engineering platform for soft tissue defect repair by combining decellularized human adipose tissue extracellular matrix (hDAM) and human adipose-derived stem cells (hASCs). To derive hDAM incised human adipose tissues underwent a decellularization process. Effective cell removal and lipid removal were proved by immunohistochemical analysis and DNA quantification. Scanning electron microscopic examination showed a three-dimensional nanofibrous architecture in hDAM. The hDAM included collagen, sulfated glycosaminoglycan, and vascular endothelial growth factor, but lacked major histocompatibility complex antigen I. hASC viability and proliferation on hDAM were proven in vitro. hDAM implanted subcutaneously in Fischer rats did not cause an immunogenic response, and it underwent remodeling, as indicated by host cell infiltration, neovascularization, and adipose tissue formation. Fresh fat grafts (Coleman technique) and engineered fat grafts (hDAM combined with hASCs) were implanted subcutaneously in nude rats. The implanted engineered fat grafts maintained their volume for 8 weeks, and the hASCs contributed to adipose tissue formation. In summary, the combination of hDAM and hASCs provides not only a clinically translatable platform for adipose tissue engineering, but also a vehicle for elucidating fat grafting mechanisms. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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