期刊
ACTA BIOMATERIALIA
卷 9, 期 7, 页码 7506-7517出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2013.03.039
关键词
Bone regeneration; Bioactive glass scaffold; Surface modification; Bone morphogenetic protein-2; Rat calvarial defect model
资金
- National Institutes of Health, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS) [1R15AR056119-01]
The repair of large bone defects, such as segmental defects in the long bones of the limbs, is a challenging clinical problem. Our recent work has shown the ability to create porous scaffolds of silicate 13-93 bioactive glass by robocasting which have compressive strengths comparable to human cortical bone. The objective of this study was to evaluate the capacity of those strong porous scaffolds with a grid-like microstructure (porosity = 50%; filament width = 330 mu m; pore width = 300 mu m) to regenerate bone in a rat calvarial defect model. Six weeks post-implantation, the amount of new bone formed within the implants was evaluated using histomorphometric analysis. The amount of new bone formed in implants composed of the as-fabricated scaffolds was 32% of the available pore space (area). Pretreating the as-fabricated scaffolds in an aqueous phosphate solution for 1, 3 and 6 days to convert a surface layer to hydroxyapatite prior to implantation enhanced new bone formation to 46%, 57% and 45%, respectively. New bone formation in scaffolds pretreated for 1,3 and 6 days and loaded with bone morphogenetic protein-2 (BMP-2) (1 mu g per defect) was 65%, 61% and 64%, respectively. The results show that converting a surface layer of the glass to hydroxyapatite or loading the surface-treated scaffolds with BMP-2 can significantly improve the capacity of 13-93 bioactive glass scaffolds to regenerate bone in an osseous defect. Based on their mechanical properties evaluated previously and their capacity to regenerate bone found in this study, these 13-93 bioactive glass scaffolds, pretreated or loaded with BMP-2, are promising in structural bone repair. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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