期刊
ACTA BIOMATERIALIA
卷 9, 期 6, 页码 6981-6991出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2013.02.014
关键词
Angiogenesis; Silicate; Bioceramic; Co-culture; Paracrine effect
资金
- National Natural Science Foundation of China [81190132, 51061160499, 31200714]
- Nature Science Foundation of Shanghai Municipal [12ZR1413900]
- State Key Laboratory of High Performance Ceramics and Superfine Structure (Shanghai Institute of Ceramics, Chinese Academy of Science) [SKL2012035IC]
Angiogenesis is critical in tissue engineering, and bioceramic-induced angiogenesis has been reported. However, the role of other types of cells such as fibroblasts in this bioceramic-induced angiogenesis process has not been reported, and is closer to the in vivo situation of tissue regeneration. In this study, the paracrine effect of silicate bioceramic-induced angiogenesis in the presence of fibroblasts was confirmed by investigating the effects of calcium silicate (CS), one of the simplest silicate bioactive ceramics, on angiogenesis in co-cultures of human dermal fibroblasts (HDF) and human umbilical vein endothelial cells (HUVEC). Results showed that CS extracts stimulated the expression of vascular endothelial growth factor (VEGF) from co-cultured HDF and subsequently enhanced the expression of VEGF receptor 2 on co-cultured HUVEC (co-HUVEC). The endothelial nitric oxide synthase and nitric oxide production in co-HUVEC was then increased to finally initiate the proangiogenesis. During this process, the expression of vascular endothelial cadherin from co-HUVEC was up-regulated, and cadherin proteins were concentrated at the cell junctions to facilitate tube formation. Silicon ions are confirmed to play an important role during silicate bioceramic-inducing angiogenesis, and effective silicon ion concentrations (0.7-1.8 mu g ml(-1)) are proposed. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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