期刊
ACTA BIOMATERIALIA
卷 8, 期 11, 页码 4130-4138出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2012.07.011
关键词
Sickle cell disease; Red blood cell; Cell biomechanics; Cell membrane; Optical measurement
资金
- National Institutes of Health at MIT [9P41-EB015871-26A1, R01HL094270, DK083242]
- KAIST, KAIST Institute for Optical Science and Technology
- Korean Ministry of Education, Science and Technology (MEST) [2009-0087691]
- National Research Foundation [NRF-2012R1A1A1009082]
- Infectious Diseases Interdisciplinary Research Group
- Singapore-MIT Alliance for Research and Technology (SMART) Center
- TJ ChungAm Foundation
Sickle cell disease (SCD) is characterized by the abnormal deformation of red blood cells (RBCs) in the deoxygenated condition, as their elongated shape leads to compromised circulation. The pathophysiology of SCD is influenced by both the biomechanical properties of RBCs and their hemodynamic properties in the microvasculature. A major challenge in the study of SCD involves accurate characterization of the biomechanical properties of individual RBCs with minimum sample perturbation. Here we report the biomechanical properties of individual RBCs from a SCD patient using a non-invasive laser interferometric technique. We optically measure the dynamic membrane fluctuations of RBCs. The measurements are analyzed with a previously validated membrane model to retrieve key mechanical properties of the cells: bending modulus; shear modulus; area expansion modulus; and cytoplasmic viscosity. We find that high cytoplasmic viscosity at ambient oxygen concentration is principally responsible for the significantly decreased dynamic membrane fluctuations in RBCs with SCD, and that the mechanical properties of the membrane cortex of irreversibly sickled cells (ISCs) are different from those of the other types of RBCs in SCD. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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