期刊
ACTA BIOMATERIALIA
卷 8, 期 8, 页码 2941-2952出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2012.04.020
关键词
Topography; Corneal endothelial cells; Endothelial cell function; Corneal regeneration; Tight-junction
资金
- National Medical Research Council [NMRC/NIG/0037/2008]
- Ministry of Education Singapore
- Division of Bioengineering in National University of Singapore
- Mechanobiology Institute Singapore
Dysfunction in the corneal endothelium, which controls the hydration and transparency of the cornea, is one of the common reasons for transplantation. A tissue-engineered corneal endothelium is of interest for corneal regeneration and for in vitro testing of ocular drugs. In the native environment, corneal endothelial cells interact with the nanotopography of the underlying Descemet's membrane. This study showed that nanotopography enhanced bovine corneal endothelial cell (BCEC) responses, creating a monolayer which resembled the healthy corneal endothelium. Topographies of different geometries were first tested to identify those that would elicit the most significant responses. A BCEC monolayer was then generated on both micro- and nanoscale pillars and wells. The BCEC monolayer cultured on topographies exhibited polygonal geometries with well-developed tight junction proteins. Scanning electron microscopy revealed that cells on pillars showed a higher density of microvilli, which was similar to native corneal endothelium. BCECs on nanopillars displayed a lower coefficient of variation of area (0.31) that was within the range of healthy corneal endothelium. More importantly, a BCEC monolayer cultured on nanopillars also had an enhanced Na+/K+-ATPase immunofluorescence expression, mRNA upregulation and a higher Na+/K+-ATPase activity. These results suggest that nanopillar substrate topography may provide relevant topographical cues, which could significantly enhance the formation and function of corneal endothelium. (c) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据