期刊
ACTA BIOMATERIALIA
卷 7, 期 11, 页码 3999-4006出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2011.06.040
关键词
Mesenchymal stem cell; Cartilage tissue engineering; Collagen; Hydroxyapatite; Osteochondral differentiation
资金
- National Natural Key Science Foundation of China [B09-B5070230]
- Key Basic Research Project of China [2011CB606204]
- National Natural Science Foundation of China [81000681, 50830101]
- Natural Science Foundation of Guangdong Province [9451064101002989]
- Fundamental Research Funds for the Central Universities [2011ZM0007]
Integrated, layered osteochondral (OC) composite materials and/or engineered DC grafts are considered as promising strategies for the treatment of OC damage. A novel biomimetic collagen-hydroxyapatite (COL-HA) OC scaffold with different integrated layers has been generated by freeze-drying. The capacity of the upper COL layer and the lower COL/HA layer to promote the growth and differentiation of human mesenchymal stem cells (hMSCs) into chondrocytes and osteoblasts respectively was evaluated. Cell viability and proliferation on COL and COL/HA scaffolds were assessed by the MTT test. The chondrogenic differentiation of hMSCs on both scaffolds was evaluated by glucosaminoglycan (GAG) quantification, alcian blue staining, type II collagen immunocytochemistry assay and real-time polymerase chain reaction in chondrogenic medium for 21 days. Osteogenic differentiation was evaluated by alkaline phosphatase activity assay, type I collagen immunocytochemistry staining, alizarin S staining and mRNA expression of osteogenic gene for 14 days in osteogenic medium. The results indicated that hMSCs on both COL and COL/HA scaffolds were viable and able to proliferate over time. The COL layer was more efficient in inducing hMSC chondrogenic differentiation than the COL/HA layer, while the COL/HA layer possessed the superiority on promoting hMSC osteogenic induction over either COL layer or pure HA. In conclusion, the layered CC composite materials can effectively promote cartilage and bone tissue generation in vitro and are potentially usable for DC tissue engineering. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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