期刊
ACTA BIOMATERIALIA
卷 7, 期 3, 页码 1339-1345出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2010.10.027
关键词
Biologic nanoparticles; Calcification; Heterotopic mineralization; Hydroxyapatite; Nanobacteria
资金
- National Institutes of Health [HL 88988]
- John E. Fetzer Memorial Trust
- Ministry of Education and Science Technology, Republic of South Korea [R13-2007-019-00000-0]
Alkaline phosphatase (ALP) is an enzyme critical for physiological and pathological biomineralization. Experiments were designed to determine whether ALP participates in the formation of calcifying nanometer sized particles (NPs) in vitro. Filtered homogenates of human calcified carotid artery, aorta and kidney stones were inoculated into cell culture medium containing 10% fetal bovine serum in the absence or presence of inhibitors of ALP or pyrophosphate. A calcific NP biofilm developed within 1 week after inoculation and their development was reduced by pyrophosphate and inhibitors of ALP. ALP protein and enzymatic activity were detected in washed NPs, whether calcified or decalcified. Therefore, ALP activity is required for the formation of calcifying NPs in vitro, as has previously been implicated during pathological calcification in vivo. (C) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据