期刊
ACTA BIOMATERIALIA
卷 6, 期 11, 页码 4285-4295出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2010.06.016
关键词
Gene vector; Biodegradable cationic polymer; PSI-g-PEI-g-PEG; Gene transfection; Cytotoxicity
资金
- National Key Basic Research Program of China [2005CB623903]
- National Natural Science Foundation of China [50633020]
Novel functional biodegradable gene vectors, poly(L-succinimide)-g-polyethylenimines-g-poly(ethylene glycol) (PSI-g-PEI-g-PEGs) were synthesized by conjugating methoxy poly(ethylene glycol) (mPEG, M-w = 750 Da) to PEI segments (M-w = 800 Da) of PSI-g-PEI. The physicochemical properties of PSI-g-PEI-g-PEGs, including buffering capability, pDNA binding ability, cytotoxicity, zeta potential and the particle size of polymer/pDNA complexes, were explored. The influence of PEGylation was discussed based on a comparative study of PSI-g-PEI-g-PEGs. PSI-g-PEI and PE125k (M-w = 25 kDa). SEM images revealed that PSI-g-PEI-g-PEG/pDNA particles have a regular shape with the diameter ranging from 70 to 170 nm. PEGylation could suppress the aggregation occurrence between complexes, resulting in a reduction of the polymer/pDNA complex size. PSI-g-PEI-g-PEGs exhibited remarkably lower cytotoxicity compared to PSI-g-PEI and PE125k. In 293T and HeLa cells, the obtained PSI-g-PEI-g-PEGs showed very high transfection efficiency compared to PEI25k. Fluorescent confocal microscopy demonstrated that PSI-g-PEI-g-PEGs could effectively transport pGL-3 plasmids into the nuclei of HeLa cells. Taking into account the continued high transfection efficacy and decreased toxicity after PEG modification, PSI-g-PEI-g-PEGs show great potential as the non-viral vectors for gene transfection. (C) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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