期刊
ACTA BIOMATERIALIA
卷 6, 期 6, 页码 2314-2321出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2009.11.034
关键词
Titanium; Surface engineering; Potassium titanate; Corrosion resistance; Mesenchymal stem cells
资金
- Natural Science Foundation of Chongqing Municipal Government [2007BA4004]
- China Ministry of Science and Technology [2009CB930000]
- Program for New Century Excellent Talents in University [NCET-07-0904]
- 111 project [B06023]
- Chongqing University [S-09104]
To improve the corrosion resistance and biological performance of commercially pure titanium (cp-Ti) substrates, potassium hydroxide was employed to modify the surfaces of titanium substrates, followed by biomimetic deposition of apatite on the substrates in a simulated body fluid. The morphologies of native and treated titanium substrates were characterized by field emission scanning electron microscopy (FE-SEM). Treatment with potassium hydroxide led to the formation of intermediate layers of potassium titanate on the surfaces of titanium substrates, while apatite was subsequently deposited onto the intermediate layer. The formation of potassium titanate and apatite was confirmed by thin-film X-ray diffraction and FE-SEM equipped with energy dispersive spectroscopy, respectively. Electrochemical impedance spectroscopy showed that the formed potassium titanate layer improved the corrosion-resistance properties of titanium substrates. The influence of modified titanium substrates on the biological behavior of mesenchymal stem cells (MSCs), including osteogenic differentiation, was investigated in vitro. Compared with cp-Ti substrates, MSCs cultured onto alkali- and heat-treated titanium substrates and apatite-deposited titanium substrates displayed significantly higher (P < 0.05 or P < 0.01) proliferation and differentiation levels of alkaline phosphatase and osteocalcin in 7 and 14 day cultures, respectively. More importantly, our results suggest that the modified titanium substrates have great potential for inducing MSCs to differentiate into osteoblasts. The approach presented here may be exploited to fabricate titanium-based implants. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据