期刊
ACTA BIOMATERIALIA
卷 5, 期 7, 页码 2560-2569出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2009.01.039
关键词
Electrospinning; Random and aligned nanofibers; PCL; Gelatin; Schwann cells
资金
- Office of Life Sciences in the National University of Singapore
- StemLife Sdn Bhd [50450]
The current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps that will produce results similar to autograft without requiring the harvest of autologous donor tissue. Aligned and random polycaprolactone/gelatin (PCL/gelatin) nanofibrous scaffolds were fabricated for the in vitro culture of Schwann cells that assist in directing the growth of regenerating axons in nerve tissue engineering. The average fiber diameter attained by electrospinning of polymer blend (PCL/gelatin) ranged from 232 +/- 194 to 160 +/- 86 nm with high porosity (90%). Blending PCL with gelatin resulted in increased hydrophilicity of nanofibrous scaffolds and yielded better mechanical properties, approaching those of PCL nanofibers. The biocompatibility of fabricated nanofibers was assessed for culturing and proliferation of Schwann cells by MTS assay. The results of the MTS assay and scanning electron microscopy confirmed that aligned and random PCL/gelatin nanofibrous scaffolds are suitable substrates for Schwann cell growth as compared to PCL nanofibrous scaffolds for neural tissue engineering. (C) 2009 Acta Materialia. Inc. Published by Elsevier Ltd. All rights reserved.
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