期刊
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
卷 45, 期 4, 页码 280-288出版社
OXFORD UNIV PRESS
DOI: 10.1093/abbs/gmt010
关键词
chicken; Krppel-like factor 7; preadipocyte; differentiation; proliferation
资金
- National Basic Research Program of China [2009CB941604]
- China Agriculture Research System [CARS-42]
- Program for Innovation Research Team in University of Heilongjiang Province [2010td02]
Krppel-like factor 7 (Klf7) has been extensively studied in the mammalian species, but its function in avian species is unclear. The objective of this study was to reveal the function of chicken Klf7 (Gallus gallus Klf7, gKlf7) in adipogenesis. The results of real-time reverse transcription polymerase chain reaction demonstrated that the relative mRNA level of chicken Klf7 (gKlf7/g-Actin) in the abdominal adipose tissue was significantly associated with the abdominal fat content and the age of broilers (P 0.05), and gKlf7 was more highly expressed in preadipocytes than in mature adipocytes (P 0.05). In addition, Oil red O staining showed that gKlf7 inhibited chicken preadipocyte differentiation, and MTT assay indicated that gKlf7 overexpression promoted preadipocyte proliferation. Additionally, luciferase assays showed that gKlf7 overexpression suppressed the chicken CCAAT/enhancer-binding protein (C/ebp), fatty acid synthase (Fasn), and lipoprotein lipase (Lpl) promoter activities (P 0.05), and gKlf7 knockdown increased the chicken peroxisome proliferator-activated receptor (Ppar), C/ebp and fatty acid-binding protein 4 (Fabp4) promoter activities (P 0.05). Together, our study demonstrated that chicken Klf7 inhibits preadipocyte differentiation and promotes preadipocyte proliferation.
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