期刊
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
卷 44, 期 8, 页码 650-659出版社
OXFORD UNIV PRESS
DOI: 10.1093/abbs/gms043
关键词
metabolomics; rice; gas chromatography; mass spectrometry; genetically modified
资金
- Basic Research on Breed Improvement of the Important Tropical Crop (Cassava) [2010CB126600]
- Genetically Modified Organisms Breeding Major Projects [2008ZX08012001]
The metabolic changes of bacterial blight-resistant line C418/Xa23 generated by molecular marker-assisted selection (n 12), transgenic variety C418-Xa21 generated by using the Agrobacterium-mediated system (n 12), and progenitor cultivar C418 (n 12) were monitored using gas chromatography/mass spectrometry. The validation, discrimination, and establishment of correlative relationships between metabolite signals were performed by cluster analysis, principal component analysis, and partial least squares-discriminant analysis. Significant and unintended changes were observed in 154 components in C418/Xa23 and 48 components in C418-Xa21 compared with C418 (P 0.05, Fold change 2.0). The most significant decreases detected (P 0.001) in both C418/Xa23 and C418-Xa21 were in three amino acids: glycine, tyrosine, and alanine, and four identified metabolites: malic acid, ferulic acid, succinic acid, and glycerol. Linoleic acid was increased specifically in C418/Xa23 which was derived from traditional breeding. This line, possessing a distinctive metabolite profile as a positive control, shows more differences vs. the parental than the transgenic line. Only succinic acid that falls outside the boundaries of natural variability between the two non-transgenic varieties C418 and C418/Xa23 should be further investigated with respect to safety or nutritional impact.
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