期刊
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
卷 43, 期 7, 页码 542-550出版社
SCIENCE PRESS
DOI: 10.1093/abbs/gmr044
关键词
biomarker; hepatocellular carcinoma; hepatitis B virus; MALDI-TOF MS
资金
- National Basic Research Program of China [2007CB936000]
- Major National Science and Technology Projects [2009ZX10004-301, 2008ZX10002-017]
- Shanghai Science and Technology Commission [10410709400, 104119 50100]
- Shanghai Talent Development Foundation [2009-035]
- National Natural Science Foundation of China [30772505, 30872503, 81000968]
Proteomic techniques are promising strategies in the surveillance of hepatocellular carcinoma (HCC). This study aimed to investigate the serum profiling with magnetic bead (MB) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and to further identify the biomarkers for HCC. Serum samples from 80 chronic hepatitis B (CHB) patients, 94 HCC concomitant with HBV patients and 24 healthy subjects were examined by MALDI-TOF MS after peptide enrichment on MBs. Based on the genetic algorithm, diagnostic models for HCC were established between 30 HCC patients and 24 healthy subjects/30 CHB patients. Validations were done with the remaining cases. Markers in the models were identified through liquid chromatography (LC)/MS-MS. The three groups were well separated from each other and two discrimination models were established for HCC. The overall recognition capability of these two models was 96.25% and 93.33%, respectively. Validations showed the misdiagnosis ratio for HCC was 1.6% and 23.4%, respectively. The identified biomarkers for HCC included prothrombin precursor (fragment), calcium-dependent secretion activator 1, Baculoviral inhibitor of apoptosis repeat-containing protein 6, etc. MB-based MALDI-TOF MS is applicable in identifying the serum biomarkers and can be used in the surveillance of HCC among HBV-infected patients.
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