期刊
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
卷 41, 期 8, 页码 648-656出版社
OXFORD UNIV PRESS
DOI: 10.1093/abbs/gmp053
关键词
gastric carcinoma; transforming growth factor-beta 1; Fascin1; RNA interference
Transforming growth factor-beta (TGF-beta) is involved in actin cytoskeleton reorganization and tumor progression. Fascin1, an actin-binding protein, increases cell invasiveness and motility in various transformed cells. To determine whether fascin1 is an important mediator of the tumor response to TGF-beta, we applied the small interfering RNA (siRNA) technique to silence fascin1 in gastric cancer (GC) cells MKN45. Results showed that the effects of TGF-beta 1 on GC cells invasion and metastasis were mediated by tumor production of fascin1; furthermore, it was found that TGF-beta 1-induced fascin1 expression was suppressed by the specific inhibitors of JNK and ERK pathways, SP6001125 and PD98059, respectively, but not by transient transfection of Smad2 and Smad4 siRNA. Our data for the first time demonstrated that fascin1 is an important mediator of TGF-beta 1-induced invasion and metastasis of GC cells, which involves JNK and ERK signaling pathways.
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