期刊
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
卷 41, 期 12, 页码 1033-1043出版社
OXFORD UNIV PRESS
DOI: 10.1093/abbs/gmp099
关键词
mangostin; reactive oxygen species; photodynamic therapy; NaN3; leukemia K562
资金
- NWU Graduate Cross-discipline Fund of Northwest University [07YJC04]
- Education Department of Shanxi Province [07JK409, 08jz67]
- National Natural Science Foundation of China [60808011]
Mangostin (MAG), a kind of xanthone widely used in diet and medicine, has antioxidant, anti-inflammatory, antimicrobial, and anticancer activities. On account of its antioxidant activity, MAG might protect cancer cells from free radical damage in photodynamic therapy (PDT) during which reactive oxygen species production was stimulated leading to irreversible tumor cell injury. In this study, the antioxidant activity of MAG was investigated and the influence of MAG on K562 cells in 5-aminolevulinic acid (ALA)-based PDT is demonstrated. The results showed that MAG could scavenge hydroxyl radical, superoxide anion, and hydrogen peroxide and inhibit the formation of malondialdehyde (MDA), but increase the amounts of singlet oxygen in cell-free systems. MAG inhibits cell proliferation and enhances cell apoptosis, lipid peroxidation, and DNA damage in ALA-PDT on K562 cells. NaN3, a singlet oxygen quencher, suppresses the MAG-induced cell apoptosis, lipid peroxidation, and DNA damage. In conclusion, MAG enhances the PDT-induced cytotoxicity in K562 cells and singlet oxygen was involved in this process. These results implied that the effect of antioxidants on PDT might be determined by its sensitization ability to singlet oxygen.
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