期刊
ACS NANO
卷 12, 期 9, 页码 9342-9354出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.8b04348
关键词
localized surface plasmon resonance (LSPR); biosensor; hydrogel; gold nanoshell; protein biomarker; dry eye; label-free
类别
资金
- National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health [R01EB022025]
- Cockrell Family Chair Foundation
- office of the Dean of the Cockrell School of Engineering at the University of Texas at Austin (UT)
- UT-Portugal Collaborative Research Program
- National Science Foundation Graduate Research Fellowships [DGE-1610403]
- Donald D. Harrington Dissertation Fellowship
- Philanthropic Educational Organization Scholar Awards program
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB022025] Funding Source: NIH RePORTER
The dependence of the localized surface plasmon resonance (LSPR) of noble-metal nanomaterials on refractive index makes LSPR a useful, label-free signal transduction strategy for biosensing. In particular, by decorating gold nanomaterials with molecular recognition agents, analytes of interest can be trapped near the surface, resulting in an increased refractive index surrounding the nanomaterial, and, consequently, a red shift in the LSPR wavelength. Ionic poly(N-isopropylacrylamide-co-methacrylic acid) (PNM) hydrogels were used as protein receptors because PNM nanogels exhibit a large increase in refractive index upon protein binding. Specifically, PNM hydrogels were synthesized on the surface of silica gold nanoshells (AuNSs). This composite material (AuNS@PNM) was used to detect changes in the concentration of two protein biomarkers of chronic dry eye: lysozyme and lactoferrin. Both of these proteins have high isoelectric points, resulting in electrostatic attraction between the negatively charged PNM hydrogels and positively charged proteins. Upon binding lysozyme or lactoferrin, AuNS@PNM exhibits large, concentration-dependent red shifts in LSPR wavelength, which enabled the detection of clinically relevant concentration changes of both biomarkers in human tears. The LSPR-based biosensor described herein has potential utility as an affordable screening tool for chronic dry eye and associated conditions.
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