期刊
ACS NANO
卷 12, 期 9, 页码 9549-9557出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.8b05270
关键词
fluorescence; naked-eye; dual-modality; immunoassay; aggregation-induced emission (AIE)
类别
资金
- Innovation and Technology Commission [ITC-CNERC14SC01, ITS/254117]
- National Science Foundation of China [21788102]
- Research Grants Council of Hong Kong [16301614, 16305015, N_HKUST604/14, A-HKUST605/16, C6009-17G]
- Science and Technology Plan of Shenzhen [JCYJ20160229205601482]
- National Natural Science Foundation of China [21705111]
- Shenzhen Science and Technology Innovation Project [JCYJ20160428142912690]
- Shenzhen Health and Family Planning Commission Project [SZBC2017004, SZSM201611064]
Sensitive and accurate detection of highly contagious virus is urgently demanded for disease diagnosis and treatment. Herein, based on a multifunctional aggregation-induced emission luminogen (AIEgen), a dual-modality readout immunoassay platform for ultrasensitive detection of viruses has been successfully demonstrated. The platform is relied on virions immunobridged enzymatic hydrolysis of AIEgen, accompanying with the in situ formation of highly emissive AIE aggregates and shelling of silver on gold nanoparticles. As a result, robust turn-on fluorescence and naked-eye discernible plasmonic colorimetry composed dual-signal is achieved. By further taking advantage of effective immunomagnetic enrichment, EV71 virions, as an example, can be specifically detected with a limit of detection down to 1.4 copies/mu L under fluorescence modality. Additionally, semiquantitative discerning of EV71 virions is realized in a broad range from 1.3 X 10(3) to 2.5 X 10(6) copies/mu L with the naked eye. Most importantly, EV71 virions in 24 real clinical samples are successfully diagnosed with 100% accuracy. Comparing to the gold standard polymerase chain reaction (PCR) assay, our immunoassay platform do not need complicated sample pretreatment and expensive instruments. This dual-modality strategy builds a good capability for both colorimetry based convenient preliminary screening and fluorescence based accurate diagnosis of suspect infections in virus-stricken areas.
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