期刊
ACS NANO
卷 8, 期 3, 页码 2410-2418出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn405963x
关键词
enzyme; DNA polymerase; catalysis; diffusion; nanomotor; chemotaxis; pump
类别
资金
- Penn State MRSEC under NSF [DMR-0820404]
- Defense Threat Reduction Agency [HDTRA1-13-1-0039]
- Pennsylvania State University Materials Research Institute Nanofabrication Lab
- National Science Foundation [ECS-0335765]
DNA polymerase is responsible for synthesizing DNA, a key component in the running of biological machinery. Using fluorescence correlation spectroscopy, we demonstrate that the diffusive movement of a molecular complex of DNA template and DNA polymerase enhances during nucleotide incorporation into the growing DNA template. The diffusion coefficient of the complex also shows a strong dependence on its inorganic cofactor, Mg2+ ions. When exposed to gradients of either nucleotide or cofactor concentrations, an ensemble of DNA polymerase complex molecules shows collective movement toward regions of higher concentrations. By immobilizing the molecular complex on a patterned gold surface, we demonstrate the fabrication of DNA polymerase-powered fluid pumps. These miniature pumps are capable of transporting fluid and tracer particles in a directional manner with the pumping speed increasing in the presence of the cofactor. The role of DNA polymerase as a micropump opens up avenues for designing miniature fluid pumps using enzymes as engines.
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