期刊
ACS NANO
卷 8, 期 6, 页码 6232-6241出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn501668a
关键词
exocytosis; gold nanoparticle; macrophage; nanotoxicity; surface chemistry
类别
资金
- National Research Foundation (NRF) - Ministry of Science, ICT & Future Planning [NRF-2012R1A1A1011058, NRF-2012M3A9C6050125]
- National R&D Program for Cancer Control, Ministry for Health and Welfare, Republic of Korea [1220070]
- Korea Health Promotion Institute [1220070] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Ministry of Science, ICT & Future Planning, Republic of Korea [KIOST01] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2012M3A9C6050124, 2012R1A1A1011058] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Significant quantities of synthetic nanoparticles circulating in the body are cleared and retained for long periods of time in the resident macrophages of the mononuclear phagocytic system (MPS), increasing the likelihood of nanoparticle-mediated chronic toxicity. To date, there has been limited effort to understand how these nanoparticles leave the macrophages. Here, we demonstrate that the native surface chemistries of gold nanoparticles (GNPs) and their subsequent opsonization by serum proteins play critical roles in the exocytosis patterns in macrophages. The cationic GNPs were retained in the cells for a relatively long time, likely due to their intracellular agglomeration. In contrast, the PEGylated GNPs migrated in the cytoplasm in the form of individual particles and exited the cells rapidly because the PEG coating mitigated interactions between GNPs and intracellular proteins. Additionally, their exocytosis pattern was not significantly governed by the size, particularly in the range from 10 to 40 nm. These results suggest that systemic excretion and toxicity of nanoparticles cleared in the MPS could be modulated by engineering their surface chemistry.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据