期刊
ACS NANO
卷 8, 期 11, 页码 11715-11723出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn5051344
关键词
short peptide self-assembly; nanofiber; hydrogel; artificial enzyme; ester hydrolysis
类别
资金
- State High-Tech Development Plan [2012AA020804]
- National Natural Science Foundation of China [30970784, 31170873, 81171455, 07165111ZX]
- National Key Basic Research Program of China [2009CB930200]
- National Distinguished Young Scholars grant [31225009]
- Chinese Academy of Sciences (CAS) Hundred Talents Program [07165111ZX]
The structural arrangement of amino acid residues in a native enzyme provides a blueprint for the design of artificial enzymes. One challenge of mimicking the catalytic center of a native enzyme is how to arrange the essential amino acid residues in an appropriate position. In this study, we designed an artificial hydrolase via self-assembly of short peptides to catalyze ester hydrolysis. When the assembled hydrolase catalytic sites were embedded in a matrix of peptide nanofibers, they exhibited much higher catalytic efficiency than the peptide nanofibers without the catalytic sites, suggesting that this well-ordered nanostructure is an attractive scaffold for developing new artificial enzymes. Furthermore, the cytotoxicity of the assembled hydrolase was evaluated with human cells, and the novel artificial biological enzyme showed excellent biocompatibility.
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