期刊
ACS NANO
卷 8, 期 2, 页码 1342-1349出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn404916f
关键词
assembly; click chemistry; conjugates; fibrils; oligonucleotides; spider silk
类别
资金
- Deutsche Forschungsgemeindschaft (DFG) [SCHE 603/4-4]
Self-assembling protein nanofibrils are promising structures for the bottom-up fabrication of bionanomaterials. Here, the recombinant protein eADF4(C16), a variant of Araneus diadematus dragline silk ADF4, which self-assembles into nanofibrils, and short oligonucleotides were modified for site-specific azide-alkyne coupling. Corresponding oligonuleotide-eADF4(C16) click conjugates were hybridized in linear or branched fashion according to the designed complementarities of the DNA moieties. Self-assembly properties of higher ordered structures of the spider silk-DNA conjugates were dominated by the silk component. Assembled beta-sheet rich conjugate fibrils were similar in appearance to fibrils of unmodified eADF4(C16) but enabled the specific attachment of neutravidin-modified gold nanoparticles on their surface directed by complementary biotin-oligonucleotides, providing the basis for functionalization of such conjugates.
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