期刊
ACS NANO
卷 8, 期 9, 页码 8887-8895出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn5034955
关键词
alpha-synuclein; controlled release; drug delivery; mesoporous silica nanoparticle; particles-on-a-particle
类别
资金
- National Research Foundation of Korean (NRF) - Ministry of Education [0458-20130016]
- Ministry of Science, ICT, and Future Planning [2013029364]
For the development of an intracellular cargo release system with mesoporous silica nanoparticles (MSN), gold nanoparticles coated with an amyloidogenic protein of alpha-synuclein were employed to prepare a protein-mediated nanocomposite into the raspberry-type particles-on-a-particle (PoP). The PoPs were successfully fabricated only at pH 4.4 by yielding the MSN coverage to 75.3% with 5 nm gold nanoparticles covalently coated with a mutant form of alpha-synuclein containing a cysteine residue at the C-terminus. The entrapped cargo of rhodamine 6G was shown to be selectively released from PoPs upon exposure to divalent cations including the alpha-synuclein-specific pathophysiological ligand of Ca2+. Intracellular uptake of the PoPs preloaded with doxorubicin as an anticancer drug and its subsequent Ca2+-dependent release were demonstrated with HeLa cells in the presence of intracellular Ca2+-regulating agents. Therefore, the fabrication of PoPs with the self-interactive protein of alpha-synuclein is expected to serve as a platform technology for preparation of diversified nanocomposites with various nanoparticles and/or bioactive molecules for eventual applications in the areas of theranostics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据