期刊
ACS NANO
卷 7, 期 3, 页码 2369-2380出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn305559j
关键词
carbon nanotubes; gelatin; hydrogel; cardiac tissue engineering; bioactuator
类别
资金
- Institute for Soldier Nanotechnology, National Institutes of Health [HL092836, EB02597, AR057837, HL099073]
- National Science Foundation [DMR0847287]
- Office of Naval Research Young Investigator award
- ONR PECASE Award
- National Research Foundation of Korea
- Korean Government [NRF-2010-220-D00014]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [0847287] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1232046] Funding Source: National Science Foundation
We engineered functional cardiac patches by seeding neonatal rat cardiomyocytes onto carbon nanotube (CNT)-incorporated photo-cross-linkable gelatin methacrylate (GelMA) hydrogels. The resulting cardiac constructs showed excellent mechanical integrity and advanced electrophysiological functions. Specifically, myocardial tissues cultured on 50 mu m thick CNT-GelMA showed 3 times higher spontaneous synchronous beating rates and 85% lower excitation threshold, compared to those cultured on pristine GelMA hydrogels. Our results indicate that the electrically conductive and nanofibrous networks formed by CNTs within a porous gelatin framework are the key characteristics of CNT-GelMA leading to Improved cardiac cell adhesion, organization, and cell-cell coupling. Centimeter-scale patches were released from glass substrates to form 3D biohybrid actuators, which showed controllable linear cyclic contraction/extension, pumping, and swimming actuations. In addition, we demonstrate for the first time that cardiac tissues cultured on CNT-GelMA resist damage by a model cardiac inhibitor as well as a cytotoxic compound. Therefore, incorporation of CNTs into gelatin, and potentially other biomaterials, could be useful in creating multifunctional cardiac scaffolds for both therapeutic purposes and In vitro studies. These hybrid materials could also be used for neuron and other muscle cells to create tissue constructs with improved organization, electroactivity, and mechanical integrity.
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