期刊
ACS NANO
卷 7, 期 12, 页码 10960-10970出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn404481f
关键词
nanoporous polymer particles; protein corona; phagocytosis; monocytes; macrophages; class A scavenger receptors
类别
资金
- Australian Research Council under the Australian Laureate Fellowship [FL120100030]
- Discovery Early Career Researcher Award [DE130100488]
- National Health and Medical Research Council (NHMRC) Program [487922]
- Australian Research Council [DE130100488] Funding Source: Australian Research Council
Many biomolecules, mainly proteins, adsorb onto polymer particles to form a dynamic protein corona in biological environments. The protein corona can significantly influence particle cell interactions, including internalization and pathway activation. In this work, we demonstrate the differential roles of a given protein corona formed in cell culture media in particle uptake by monocytes and macrophages. By exposing disulfide-stabilized poly-(methacrylic acid) nanoporous polymer particles (PMA(SH) NPPs) to complete cell growth media containing 10% fetal bovine serum, a protein corona, with the most abundant component being bovine serum albumin, was characterized. Upon adsorption onto the PMA(SH) NPPs, native bovine serum albumin (BSA) was found to undergo conformational changes. The denatured BSA led to a significant decrease in internalization efficiency in human monocytic cells, THP-1, compared with the bare particles, due to reduced cell membrane adhesion. In contrast, the unfolded BSA on the NPPs triggered class A scavenger receptor-mediated phagocytosis in differentiated macrophage-like cells (dTHP-1) without a significant impact on the overall internalization efficiency. Taken together, this work demonstrates the disparate effects of a given protein corona on particle-cell interactions, highlighting the correlation between protein corona conformation in situ and relevant biological characteristics for biological functionalities.
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