期刊
ACS NANO
卷 7, 期 6, 页码 5568-5576出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn401837q
关键词
fluorescence-activatable probe; gold nanoparticle; prostate-specific antigen (PSA); rhodamine B isothiocyanate (RBITC)
类别
资金
- Intramural Research Program (IRP) of the National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH)
- NIH-NIBIB/NIST NRC
It Is still in high demand to develop extremely sensitive and accurate clinical tools for biomarkers of interest for early diagnosis and monitoring of diseases. In this report, we present a highly sensitive and compatible gold nanoparticle (AuNP)-based fluorescence-activatable probe for sensing ultralow levels of prostate-specific antigen (PSA) in patient serum samples. The limit of detection of the newly developed probe for PSA was pushed down to 0.032 pg/mL, which is more than 2 orders of magnitude lower than that of the conventional fluorescence probe. The ultrahigh sensitivity of this probe was attributed to the high loading efficiency of the dyes on AuNP surfaces and high fluorescence quenching-unquenching abilities of the dye-AuNP pairs. The efficiency and robustness of this probe were investigated In patient serum samples, demonstrating the great potential of this probe in real-world applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据