期刊
ACS NANO
卷 7, 期 9, 页码 7500-7512出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn401095p
关键词
nanobiotechnology; magnetic resonance imaging; biomaterials; tissue engineering; stem cells
类别
资金
- French Research Funding Agency (ANR) [ANR-11 SVSE5]
- NMVASC [ANR-10 INTB-1502-01]
- European Project Magnifyco [NMP4-SL-2009-228622]
- Agence Nationale de la Recherche (ANR) [ANR-10-INTB-1502] Funding Source: Agence Nationale de la Recherche (ANR)
Recent advances in cell therapy and tissue engineering opened new windows for regenerative medicine, but still necessitate innovative noninvasive imaging technologies. We demonstrate that high-resolution magnetic resonance imaging (MRI) allows combining cellular-scale resolution with the ability to detect two cell types simultaneously at any tissue depth. Two contrast agents, based on iron oxide and gadolinium oxide rigid nanoplatforms, were used to tattoo endothelial cells and stem cells, respectively, with no impact on cell functions, including their capacity for differentiation. The labeled cells' contrast properties were optimized for simultaneous MRI detection: endothelial cells and stem cells seeded together in a polysaccharide-based scaffold material for tissue engineering appeared respectively in black and white and could be tracked, at the cellular level, both in vitro and in vivo. In addition, endothelial cells labeled with iron oxide nanoparticles could be remotely manipulated by applying a magnetic field, allowing the creation of vessel substitutes with in depth detection of individual cellular components.
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