期刊
ACS NANO
卷 7, 期 5, 页码 4617-4628出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn401424y
关键词
electron beam lithography; quantum dots; micropattern arrays; neutrophils; live cell imaging
类别
资金
- Federal Ministry of Education and Research (BMBF) [FKZ 03Z2CN11]
Quantum dot (QD) based micro-/nanopatterned arrays are of broad interest in applications ranging from electronics, photonics, to sensor devices for biomedical purposes. Here, we report on a rapid, physico-chemically mild approach to generate high fidelity micropattem arrays of prefunctionalized water-soluble quantum dots using electron beam lithography. We show that such patterns retain their fluorescence and bioaffinity upon electron beam lithography and, based on the streptavidin biotin interaction, allow for detection of proteins, colloidal gold nanoparticles and magnetic microparticles. Furthermore, we demonstrate the applicability of QD based microamay patterns differing in their shape (drdes, squares, grid-like), size (from 1 to 10 /mu n) and pitch distance to study the adhesion, spreading and migration of human blood derived neutrophils. Using live cell confocal fluorescence microscopy, we show that pattem geometry and pitch distance influence the adhesion, spreading and migratory behavior of neutrophils. Research reported in this work paves the way for produdng QD microarrays with multiplexed functionalities relevant for applications in analyte sensing and cellular dynamics.
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