期刊
ACS NANO
卷 7, 期 4, 页码 2948-2959出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn304868y
关键词
silver nanoparticles; drug delivery; photoactivation; antisense therapeutics; nanomedicine
类别
资金
- National Science Foundation [CBET-0748195, CMMI-0963482]
- State of Louisiana Board of Regents Support Fund
- LSU Economic Development Assistantship
- Donald Clayton Graduate Fellowship
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [748195] Funding Source: National Science Foundation
The unique photophysical properties of noble metal nanoparticles contribute to their potential as photoactivated drug delivery vectors. Here we demonstrate the synthesis and characterization of 60-80 nm silver nanoparticles (SNPs) decorated with thiol-terminated photolabile DNA oligonucleotides. In vitro assays and fluorescent confocal microscopy of treated cell cultures show efficient UV-wavelength photoactivation of surface-tethered caged 15152302 antisense oligonucleotides possessing internal photocleavable linkers. As a demonstration of the advantages of these novel nanocarriers, we investigate properties including: enhanced stability to nucleases, increased hybridization activity upon photorelease, and efficient cellular uptake as compared to commercial transfection vectors. Their potential as multicomponent delivery agents for oligonucleotide therapeutics is shown through regulation of ICAM-1 (Intracellular Adhesion Molecule-1) silencing. Our results suggest a means to achieve light-triggered, spatiotemporally controlled gene silencing via nontoxic silver nanocarriers, which hold promise as tailorable platforms for nanomedicine, gene expression studies, and genetic therapies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据