期刊
ACS NANO
卷 6, 期 3, 页码 2731-2740出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn300172t
关键词
amine-modified graphene; graphene oxide; thrombus; surface charge distribution; hemolysis; thromboembolism
类别
资金
- Department of Biotechnology (DBT)
- Department of Science and Technology (DST), Government of India
- Indian Council of Medical Research (ICMR)
- University Grants Commission
- ICMR, New Delhi
- Institute of Medical Sciences, BHU
Graphene and its derivatives have attracted significant research interest based on their application potential in different fields including biomedicine. However, recent reports from our laboratory and elsewhere have pointed to serious toxic effects of this nanomaterial on cells and organisms. Graphene oxide (GO) was found to be highly thrombogenic in mouse and evoked strong aggregatory response in human platelets. As platelets play a central role in hemostasis and thrombus formation, thrombotoxicity of GO potentially limits its biomedical applications. Surface chemistry of nanomaterials is a critical determinant of biocompatibility, and thus differentially functionalized nanomaterials exhibit varied cellular toxicity. Amine-modified carbon nanotubes have recently been shown to possess cytoprotective action, which was not exhibited by their relatively toxic carboxylated counterparts. We, therefore, evaluated the effect of amine modification of graphene on platelet reactivity. Remarkably, our results revealed for the first time that amine-modified graphene (G-NH2) had absolutely no stimulatory effect on human platelets nor did it induce pulmonary thromboembolism in mice following Intravenous administration. Further, it did not evoke lysis of erythrocytes, another major cellular component in blood. These findings contrasted strikingly the observations with GO and reduced GO (RGO). We conclude that G-NH2 is not endowed with thrombotoxic property unlike other commonly investigated graphene derivatives and is thus potentially safe for in vivo biomedical applications.
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