期刊
ACS NANO
卷 6, 期 4, 页码 2978-2983出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn300684d
关键词
graphene; chemiluminescence; CRET; immunosensors; C-reactive protein
类别
资金
- National Research Foundation (NRF) via NLRL [2011-0028915]
- Converging Research Center [2009-0082276]
- National Research Laboratory [R0A-2008-000-20041-0]
- Intelligent Synthetic Biology Center of Global Frontier Project [2011-0031957]
- Engineering Research Center [2012-0001175]
- National Research Foundation of Korea [2011-0028915] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We report on chemiluminescence resonance energy transfer (CRET) between graphene nanosheets and chemiluminescent donors. In contrast to fluorescence resonance energy transfer, CRET occurs via nonradiative dipole dipole transfer of energy from a chemiluminescent donor to a suitable acceptor molecule without an external excitation source. We designed a graphene-based CRET platform for homogeneous immunoassay of C-reactive protein (CRP), a key marker for human inflammation and cardiovascular diseases, using a luminol/hydrogen peroxide chemiluminescence (CL) reaction catalyzed by horseradish peroxidase. According to our results, anti-CRP antibody conjugated to graphene nanosheets enabled the capture of CRP at the concentration above 1.6 ngmL(-1). In the CRET platform, graphene played a key role as an energy acceptor, which was more efficient than graphene oxide, while luminol served as a donor to graphene, triggering the CRET phenomenon between luminol and graphene. The graphene-based CRET platform was successfully applied to the detection of CRP in human serum samples In the range observed during acute inflammatory stress.
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