期刊
ACS NANO
卷 6, 期 3, 页码 2582-2590出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn2050449
关键词
molecular beacons; silicon nanowires; gold nanoparticles; DNA; multianalysis
类别
资金
- National Basic Research Program of China (973 Program) [2012CB-932400, 2012CB932600]
- NSFC [30900338, 51072116]
- Research Grants Council of Hong Kong SAR, China [CityU5/CRF/08, CityU102010]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Nanomaterial-based molecular beacons (nanoMBs) have been extensively explored due to unique merits of nanostructures, including gold nanoparticle (AuNP)-, carbon nanotube (CNT)-, and graphene-based nanoMBs. Those nanoMBs are well-studied; however, they possess relatively poor salt stability or low specificity, limiting their wide applications. Here, we present a novel kind of multicolor silicon-based nanoMBs by using AuNP-decorated silicon nanowires as high-performance quenchers. Significantly, the nanoMBs feature robust stability In high-concentration (0.1 M) salt solution and wide-ranging temperature (10-80 degrees C), high quenching efficiency (>90%) for various fluorophores (e.g., FAM, Cy5, and ROX), and large surfaces for simultaneous assembly of different DNA strands. We further show that silicon-based nanoMBs are highly effective for sensitive and specific multidetection of DNA targets. The unprecedented advantages of silicon-based multicolor nanoMBs would bring new opportunities for challenging bioapplications, such as allele discrimination, early cancer diagnosis, and molecular engineering, etc.
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