期刊
ACS NANO
卷 6, 期 7, 页码 5961-5971出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn300868z
关键词
superparamagnetic iron oxide nanoparticles; photothermal imaging; cellular uptake of nanoparticles; membrane binding of nanoparticles; cryosections
类别
资金
- EPSRC [EP/H046143/1]
- Marie Curie EU [PERG08-GA-2010-276909]
- Engineering and Physical Sciences Research Council [EP/H046143/1] Funding Source: researchfish
- EPSRC [EP/H046143/1] Funding Source: UKRI
A detailed understanding of cellular interactions with superparamagnetic iron oxide nanoparticles (SPIONs) is critical when their biomedical applications are considered. We demonstrate how photothermal microscopy can be used to follow the cellular uptake of SPIONs by direct imaging of the iron oxide core. This offers two important advantages when compared with current strategies employed to image magnetic cores: first, it is nondestructive and is therefore suitable for studies of live cells and, second, it offers a higher sensitivity and resolution, thus allowing for the identification of low levels of SPIONs within a precise subcellular location. We have shown that this technique may be applied to the imaging of both cell monolayers and cryosections. In the former we have demonstrated the role of temperature on the rate of endocytosis, while in the latter we have been able to identify cells labeled with SPIONs from a mixed population containing predominantly unlabeled cells. Direct imaging of the SPION core is of particular relevance for research involving clinically approved SPIONs, which do not contain fluorescent tags and therefore cannot be detected via fluorescence microscopy.
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