期刊
ACS NANO
卷 6, 期 3, 页码 2712-2721出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn300147e
关键词
DNA polymorphisms; molecular brushes; biosensors; heat-transfer resistance; impedance spectroscopy; confocal fluorescence microscopy; nanocrystalline CVD diamond
类别
资金
- FWO-Research Foundation Flanders [G.0829.09]
- Belgian Interuniversity Attraction Pole Programme IAP-VI
- BOF of Hasselt University
- Life-Sciences Impulse Programme of the Belgian Province of Limburg
- IWT-Agency for Innovation by Science and Technology
In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer resistance at the solid-to-liquid Interface. In contrast to this, molecular brushes of single-stranded DNA cause, surprisingly, a substantially higher heat-transfer resistance and behave like a thermally insulating layer. This effect can be utilized to identify ds-DNA melting temperatures via the switching from low- to high heat-transfer resistance. The melting temperatures identified with this method for different DNA duplexes (29 base pairs without and with built-in mutations) correlate nicely with data calculated by modeling. The method is fast, label-free (without the need for fluorescent or radioactive markers), allows for repetitive measurements, and can also be extended toward array formats. Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA.
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