期刊
ACS NANO
卷 6, 期 5, 页码 4094-4103出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn3004923
关键词
human embryonic stem cell; nanotopography; microfabrication; mechanosensitivity; self-renewal
类别
资金
- National Science Foundation [CMMI 1129611]
- National Institute of Health [UL1RR024986, R01 DE016530, 4R00 CA136759-02]
- Alzheimer's Association
- Department of Mechanical Engineering
- NIDCR at the University of Michigan School of Dentistry, Ann Arbor
- Directorate For Engineering [1129611] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn [1129611] Funding Source: National Science Foundation
Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughnes on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell-matrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type specific, and as such, we could generate a spatially segregated coculture system for hESCs and NIH/3T3 fibroblasts using patterned nanorough glass surfaces.
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