期刊
ACS NANO
卷 6, 期 12, 页码 10776-10785出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn304101x
关键词
supramolecular nanofibers; cell morphological analysis; nitrobenzyl; projected cell area; photodegradable; RGDS epitope
类别
资金
- NIDCR [2R01DE015920-06]
- NIBIB [2R01EB003806-06A2]
- National Institutes of Health [1F32AR061955-01]
- U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-AC02-06CH11357]
Self-assembling peptide materials have been used extensively to mimic natural extracellular matrices (ECMs) by presenting bioactive epitopes on a synthetic matrix. Although this approach can facilitate a desired response from cells grown in the matrix, it lacks the capacity for spatial or temporal regulation of the presented signals. We describe here a photoresponsive, synthetic ECM using a supramolecular platform composed of peptide amphiphiles (PAs) that self-assemble into cylindrical nanofibers. A photocleavable nitrobenzyl ester group was included in the peptide backbone using a novel Fmoc-amino acid that is compatible with microwave-assisted solid-phase peptide synthesis. The placement of the photolabile group on the peptide backbone enabled efficient removal of the ECM-derived cell adhesion epitope RODS from PA molecules upon exposure to light (half-life of photolysis similar to 1.9 min) without affecting the nanofiber assembly. Fibroblasts cultured on RODS-presenting PA nanofiber substrates demonstrated increased cell spreading and more mature focal adhesions compared with unfunctionalized and control (ROES-presenting) surfaces, as determined by immunostaining and cell morphological analysis. Furthermore, we observed an arrest in fibroblast spreading on substrates containing a cleavable RODS epitope when the culture was exposed to light; in contrast, this dynamic shift in cell response was absent when the RODS epitope was attached to the PA molecule by a light-insensitive control linker. Light-responsive bioactive materials can contribute to the development of synthetic systems that more closely mimic the dynamic nature of native ECM.
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