期刊
ACS NANO
卷 5, 期 9, 页码 6935-6944出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn201266e
关键词
anodic aluminum oxide; fluorescence microscopy; fluorescence recovery after photobleaching; molecular encapsulation; nanoporous substrates; silane chemistry
类别
资金
- DFG [JA 963/8, STE 884/9-1 SFB 803]
- Fonds Quebecois de Recherche sur la Nature et les Technologies (FQRNT)
- Gottingen Graduate School for Neurosciences and Molecular Biosciences (GGNB)
- MPIP-IMPRS Mainz
- IMPRS-PBCS Gottingen
Anodic aluminum oxide (AAO) is a porous material having aligned cylindrical compartments with 55-60 nm diameter pores, and being several micrometers deep. A protocol was developed to generate pore-spanning fluid lipid bilayers separating the attoliter-sized compartments of the nanoporous material from the bulk solution, while preserving the optical transparency of the AAO. The AAO was selectively functionalized by silane chemistry to spread giant unilamellar vesicles (GUVs) resulting In large continuous membrane patches covering the pores. Formation of fluid single lipid bilayers through GUV rupture could be readily observed by fluorescence microscopy and further supported by conservation of membrane surface area, before and after GUV rupture. Fluorescence recovery after photobleaching gale low immobile fractions (5-15%) and lipid diffusion coefficients similar to those found for bilayers on silica. The entrapment of molecules within the porous underlying cylindrical compartments, as well as the exclusion of macromolecules from the nanopores, demonstrate the barrier fun ton of the pore-spanning membranes and could be investigated in three-dimensions using confocal laser scanning fluorescence imaging.
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