期刊
ACS NANO
卷 5, 期 7, 页码 5864-5873出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn201643f
关键词
nanosensors; pH measurements; intracellular; nanoparticles; endosome; lysosome; fluorescence microscopy
类别
资金
- Kraeftens Bekaempelse, Denmark
- Danish Council for Independent Research (Technology and Production Sciences (FTP)) [(LiMeS) 274-07-0172]
Particle-based nanosensors have over the past decade been designed for optical fluorescent-based ratiometric measurements of pH in living cells. However, quantitative and time-resolved intracellular measurements of pH in endosomes and lysosomes using particle nanosensors are challenging, and there is a need to improve measurement methodology. In the present paper, we have successfully carried out time-resolved pH measurements in endosomes and lyosomes in living cells using nanoparticle sensors and show the importance of sensor choice for successful quantification. We have studied two nanoparticle-based sensor systems that are internalized by endocytosis and elucidated important factors in nanosensor design that should be considered In future development of new sensors. From our experiments it is dear that it is highly Important to use sensors that have a broad measurement range, as erroneous quantification of pH is an unfortunate result when measuring pH too close to the limit of the sensitive range of the sensors. Triple-labeled nanosensors with a pH measurement range of 3.2-7.0, which was synthesized by adding two pH-sensitive fluorophores with different pK(a) to each sensor, seem to be a solution to some of the earlier problems found when measuring pH In the endosome-lysosome pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据