期刊
ACS NANO
卷 5, 期 4, 页码 2870-2879出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn103476x
关键词
nanorods; surface chemistry; cytotoxicity; gene expression; inflammation; cellular uptake
类别
资金
- NSF [0906838]
- Oak Ridge Institute for Science and Education
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [906838] Funding Source: National Science Foundation
Gold nanorods (GNRs) stabilized with cetyltrimethylammonium bromide (CTAB) and GNR functionalized via a ligand exchange method with either thiolated polyethylene glycol (PEG(5000)) or mercaptohexadecanoic acid (MHDA) were investigated for their stability in biological media and subsequent toxicological effects to HaCaT cells. GNR-PEG and GNR-MHDA exhibited minimal effects on cell proliferation, whereas GNR-CTAB reduced cell proliferation significantly due to the inherent toxicity of the cationic surfactant to cells. Cell uptake studies indicated relatively IOW uptake for GNR-PEG and high uptake for GNR-MHDA. Reverse transcriptase polymerase chain reaction (RT-PCR) revealed that GNR-PEG induced less significant and unique changes in the transcription levels of 84 genes related to stress and toxicity compared to GNR-MHDA. The results demonstrate that, although cell proliferation was not affected by both particles, there is a significant difference In gene expression in GNR-MHDA exposed cells, suggesting long-term implications for chronic exposure.
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