期刊
ACS NANO
卷 5, 期 6, 页码 4512-4520出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn103557s
关键词
TMV-like particles; bottom-up assembly; nanotube array; biotemplate; Si wafer; polymer blend lithography
类别
资金
- Baden-Wuerttemberg-Stiftung, Network of Competence Functional Nanostructures
- DFG [SPP1165]
- CFN
Tobacco mosaic virus (TMV) is a tube-shaped, exceptionally stable plant virus, which is among the biomolecule complexes offering most promising perspectives for nanotechnology applications. Every viral nanotube self-assembles from a single RNA strand and numerous identical coat protein (CP) subunits. Here we demonstrate that biotechnologically engineered RNA species containing the TMV origin of assembly can be selectively attached to solid surfaces via one end and govern the bottom-up growth of surface-linked TMV-like nanotubes in situ on demand. 5032 wafers patterned by polymer blend lithography were modified in a chemically selective manner, which allowed positioning of in vitro produced RNA scaffolds into predefined patches on the 100-500 nm scale. The RNA operated as guiding strands for the self-assembly of spatially ordered nanotube 3D arrays on the micrometer scale. This novel approach may promote technically applicable production routes toward a controlled integration of multivalent biotemplates into miniaturized devices to functionalize poorly accessible components prior to use. Furthermore, the results mark a milestone In the experimental verification of viral nucleoprotein complex self-assembly mechanisms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据