期刊
ACS NANO
卷 6, 期 1, 页码 760-770出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn2042384
关键词
carbon nanotubes; graphene; carbohydrate; lectin; sensors
类别
资金
- NIEHS [R01ES019304]
- Bayer MaterialScience
- EPA STAR [FP-91713801]
- Universite Claude Bernard Lyon
- CNRS
- Region Rhone-Alpes (Cluster de Recherche Chimie)
- ANR Glycoasterix
- Vaincre la Mucoviscidose
- GDR Pseudomonas
Here we investigated the interactions between lectins and carbohydrates using field-effect transistor (FET) devices comprised of chemically converted graphene (CCG) and single-walled carbon nanotubes (SWNTs). Pyrene- and porphyrin-based glycoconjugates were functionalized noncovaiently on the surface of CCG-FET and SWNT-FET devices, which were then treated with 2 pm nonspecific and specific lectins. In particular, three different lectins (PA-IL, PA-IIL, and ConA) and three carbohydrate epitopes (galactose, fucose, and mannose) were tested. The responses of 36 different devices were compared and rationalized using computer-aided models of carbon nanostructure/glycoconjugate interactions. Glycoconjugate surface coverage in addition to one-dimensional structures of SWNTs resulted In optimal lectin detection. Additionally, lectin titration data of SWNT- and CCG-based biosensors were used to calculate lectin dissociation constants (K-d) and compare them to the values obtained from the isothermal titration microcalorimetry technique.
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