期刊
ACS NANO
卷 5, 期 2, 页码 693-729出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn103298x
关键词
single molecule; scanning tunneling microscopy; atomic force microscopy; transmission electron microscopy; optical microscopy; subdiffraction microscopy; Forster resonance energy transfer; fluorescence; photoactivation light microscopy; magnetic resonance; spin; magnetic resonance force microscopy; electron spin resonance; nitrogen vacancy
类别
资金
- DoE [DE-SC00-05161]
- NSF [NSF-CHE-1041943]
- NIH (NASA)
- Kavli Foundation
Single-molecule measurement techniques have illuminated unprecedented details of chemical behavior, including observations of the motion of a single molecule on a surface, and even the vibration of a single bond within a molecule. Such measurements are critical to our understanding of entities ranging from single atoms to the most complex protein assemblies. We provide an overview of the strikingly diverse classes of measurements that can be used to quantify single-molecule properties, including those of single macromolecules and single molecular assemblies, and discuss the quantitative insights they provide. Examples are drawn from across the single-molecule literature, ranging from ultrahigh vacuum scanning tunneling microscopy studies of adsorbate diffusion on surfaces to fluorescence studies of protein conformational changes in solution.
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