期刊
ACS NANO
卷 5, 期 10, 页码 7848-7857出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn201904t
关键词
single-walled carbon nanotube; near-infrared fluorescence; single-molecule detection; hydrogen peroxide; redox signaling; angiogenesis; europium(III) hydroxide nanorods
类别
资金
- Beckman Young Investigator Award
- National Science Foundation
- Korea Research Foundation
- Korean Government (MOEHRD) [KRF-2007-357-000086]
- DST, Government of India, New Delhi [GAP 0305\DST\CP]
- National Institutes of Health (NIH) [HL70567, CA150190]
- Center for Environmental Health and Science at MIT
Reactive oxygen species, specifically hydrogen peroxide (H2O2), activate signal transduction pathways during angiogenesis and therefore play an Important role in physiological development as well as various pathophysiologies. Herein, we utilize a near-Infrared fluorescent single-walled carbon nanotube (SWNT) sensor array to measure the single-molecule efflux of H2O2 from human umbilical vein endothelial cells (HUVEC) in response to angiogenic stimulation. Two angiogenic agents were investigated: the pro-angiogenic cytokine, vascular endothelial growth factor A (VEGF-A) and the recently identified inorganic pro-angiogenic factor, europium(III) hydroxide in nanorod form. The nanosensor array consists of a SWNT embedded within a collagen matrix that exhibits high selectivity and sensitivity to single molecules of H2O2. A calibration from 123 to 400 nM quantifies the production of H2O2 at nanomolar concentration in HUVEC with 1 s temporal and 300 nm spatial resolutions. We find that the production of H2O2 following VEGF stimulation is elevated outside of HUVEC, but not for stimulation via nanorods, while increased generation is observed in the cytoplasm for both cases, suggesting two distinct signaling pathways.
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