期刊
ACS NANO
卷 5, 期 10, 页码 8230-8240出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn202912b
关键词
imaging agents; nanostructures; polyelectrolyte; magnetic resonance imaging; near-infrared imaging; cancer cells
类别
资金
- National Research Foundation of Korea (NRF)
- Korea government (MEST) [2010-0026793]
- National Research Facilities & Equipment Centre
- Korea Research Council of Fundamental Science and Technology
- Korea Basic Science Institute [T31403]
- National Research Foundation of Korea
- National Research Foundation of Korea [2010-0026793] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Here, we describe an easy but robust chemical strategy to synthesize high-performance magnetic resonance (MR)/near-infrared (NIR) multimodal imaging nanoprobes. Poly(gamma-glutamic acid) was used for the convenient phase transfer of MnFe2O4 nanoparticles dispersed in organic solvents into aqueous solutions and facilitated further Ionic gelation with poly(L-lysine). During the gelation process, MnFe2O4 nanoparticulate satellites were encapsulated in the ionic nanocomplex, which Induced synergistic magnetism and resulted in huge T-2 relaxivity (r(2)). The positively charged outer surfaces were assembled with other negatively charged NIR emitting fluorescent nanocrystals and enabled the highly efficient delivery of the magnetofluorescent polyelectrolyte nanocomposites (MagFL-PEN) into cancer cells. The enhancement of negative contrast of MagFL-PEN at 2 mu g/mL concentration was similar to that of Resovist at 20 mu g/mL concentration. The NIR fluorescence microscopy images of the MagFL-PEN-labeled cells even at 12.5 pM were able to be clearly observed. The labeling efficiency of MagFL-PEN was approximately 65-fold higher compared to that of the commercialized fluorescent nanocrystals, only after 3 h incubation period, even at the test concentration (100 pM). Due to the high-performance capabilities both in materials properties and cell labeling efficiency, the MagFL-PEN is expected to be used as a highly efficient MR/NIR dual-modality imaging nanoprobe In the detection of cancer cells and monitoring of therapeutic cells in vivo.
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